Coding

Part:BBa_K773002:Design

Designed by: Katie Knister   Group: iGEM12_Caltech   (2012-10-01)

Proteorhodopsin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 515
    Illegal XhoI site found at 88
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 277
    Illegal AgeI site found at 652
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We codon optimized the gene for E. coli and eliminated four restriction sites. Our characterization assays of this part were inconclusive, but the sequencing data shows that this is proteorhodopsin. To view the latest characterization assay go to the [http://2012.igem.org/Team:Caltech/Parts/Characterization Caltech Characterization] page.


Source

This part was synthesized by Daisy Lin, Chenxi Qiu, Emzo de los Santos, Nate Glasser, Edward Pursifull, and Katie Knister with [http://2012.igem.org/Team:Caltech/Notebook/material/General_Protocols#Gene_Assembly gene assembly] using the information from the paper [http://www.pnas.org/content/104/7/2408.abstract Light-powering Escherichia coli with proteorhodopsin].

References

[http://www.pnas.org/content/104/7/2408.abstract Light-powering Escherichia coli with proteorhodopsin]
[http://helixweb.nih.gov/dnaworks/ DNAWorks] (to obtain oligonucleotides to assemble the gene)